Table 1 Primer sequences and thermal cycling conditions of QacE∆1 and CepA resistance genes
Gene | Primer sequence (5’ → 3’) | Thermal cycling conditions | Amplicon size (bp) | |||||
|---|---|---|---|---|---|---|---|---|
Initial denaturation (°C/time) | Denaturation (°C/time) | Annealing (°C/ time) | Cycles | Extension (°C/time) | Final extension | |||
qacE∆1 | F: AATCCATCCCTGTCGGTGTT R: CGCAGCGACTTCCACGATGGGGAT | 94 /5 min | 94/30 sec. | 53/45 sec. | 30 | 72/50 sec. | 72/10 min. | 190 |
cepA | F: CAACTCCTTCGCCTATCCCG R: TCAGGTCAGACCAAACGGCG | 94/5 min. | 94/30 sec. | 66/45 sec. | 30 | 72/50 sec. | 72/10 min. | 1051 |