Fig. 2

Temporal phylogenetic reconstruction of ST764 MRSA lineage. (A) Bayesian phylogenetic reconstruction of the ST764 MRSA lineage using 1,684 non-recombinant core genome SNPs from 97 isolates (14 from this study). Isolates are represented by dots at the tree tips, colored by isolation country. Strain names of EIA-MRSA isolates are colored in red. Heatmaps show the presence of QRDR mutations and the TSST-1 virulence factor, with colored cells indicating presence and white cells indicating absence. (B) Linear regression plot showing the relationship between date (x-axis) and root-to-tip divergence (y-axis), used to assess the correspondence between the phylogenetic signal (core genome SNPs-based maximum likelihood phylogeny of the ST764 MRSA lineage) and temporal signal (isolation date). (C) Bayesian skyline plot showing changes in the effective population size of the ST764 MRSA population (n = 97) over time. The estimated variations are represented by a blue line, with 95% confidence intervals depicted by a blue shaded area