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Table 1 Collection points for water and snail samples in the district of Giru, Joaíma, and the municipality of Ribeirão Das Neves, in Minas Gerais, Brazil, including the abiotic parameters measured, the concentration of Escherichia coli and total coliforms in water bodies

From: Advancing schistosomiasis surveillance: standardization and application of an environmental DNA (eDNA)-based approach for detecting Schistosoma mansoni in Brazil

Collection point

Geographic coordinates

Taxonomy identification

Parasitological exam

Number of collected snails

Number of positive snails

Water temperature

°C

pH

Calcium

(mg/L)

Conductivity

(µS/cm)

E.coli (CFU 100 mL− 1)

Total Coliforms (CFU 100 mL− 1)

Literature*

 

18 to 31

5.6 to 9.1

2.4 to 129

 
 

Giru (First survey)

 

1

-41.053185

-16.861399

Dead

-

6

0

NP

NP

NP

NP

NP

NP

2

-41.051812

-16.860166

Dead

-

2

0

NP

NP

NP

NP

NP

NP

3

-41.065900

-16.866780

B. glabrata

S. mansoni

3

1

NP

NP

NP

NP

NP

NP

4

-41.057031

-16.864323

B. glabrata

-

32

0

NP

NP

NP

NP

NP

NP

5

-41.044322

-16.872878

B. glabrata

-

3

0

NP

NP

NP

NP

NP

NP

 

Giru (Second survey)

 

4

-41.05715

-16.864323

B. glabrata

-

54

3*

31.97 ± 0.29

6.85 ± 0.07

4.33 ± 0.58

87.33 ± 1.53

5,1 × 102 ± 7.50 log

3.6 × 103 ± 5.5 log

6

-41.06278

-16.86698

B. glabrata

S. mansoni

322

4**

27.77 ± 0.55

7.40 ± 0.20

4.00 ± 0.00

83.67 ± 4.16

8.2 × 103 ± 3.0 log

10 × 103 ± 0 log

7

-41.07110

-16.86609

-

-

0

-

25.60 ± 0.10

7.37 ± 0.15

4.00

24.33 ± 1.53

5.5 × 102 ± 7.1 log

10 × 103 ± 0 log

8

-41.05881

-16.8603

B. glabrata

-

6

0

27.63 ± 0.25

6.83 ± 0.06

11.00 ± 1.00

93.33 ± 4.51

5 × 101 ± 0.8 log

6.8 × 103 ± 5.4 log

9

-41.06615

-16.86483

-

-

0

-

30.47 ± 1.20

7.60

7.13 ± 0.06

28.67 ± 2.08

5,2 × 103 ± 6.7 log

6.8 × 103 ± 5.4 log

 

Ribeirão das Neves

 

1

-44.114034

-19.770934

B. straminea

-

24

0

29.57 ± 1.07

6.97 ± 0.12

39.33 ± 1.53

108.00 ± 4.36

5.4 × 102±4.1 log

4,3 × 102±1.1 log

2

-44.110362

-19.786881

-

-

0

-

35.17 ± 1.23

6.83 ± 0.12

15.33 ± 0.58

62.00 ± 5.57

4.0 × 104 ±2.4 log

2.1 × 104 ±1.0 log

3

-44.016439

-19.813047

B. tenagophila

-

134

0

33.13 ± 0.85

7.47 ± 0.06

46.00 ± 1.73

437.00 ± 57,58

2.8 × 104 ±1.8 log

6.1 × 104±6.5 log

  1. NP: Not performed
  2. * Three snails releasing cercariae with a long, single-tailed, eyeless body and elongated shape, identified as belonging to the Echinostomatidae family by Multiplex PCR
  3. ** One snail releasing S. mansoni cercaria, confirmed by molecular techniques; and three snails releasing cercariae with a long, single-tailed, eyeless body and elongated shape, identified as belonging to the Echinostomatidae family by Multiplex PCR
  4. Ministério da Saúde, 2008 and ┼┼Brown et al. (1994)