Fig. 2

Poly(A) length distribution and Gene Set Enrichment Analysis (GSEA) using genes ranked by poly(A) tail lengths. A Poly(A) length distribution in mitochondrial transcripts. B Poly(A) length distribution in nuclear transcripts. C-E Ridgeplots from the clusterProfiler package with the X-axis indicating the poly(A) lengths and Y-axis indicating the GO term or KEGG pathway. The distribution is the distribution of poly(A) length of those genes that enriched in the corresponding GO enrichment analysis C molecular function D cellular component and E KEGG pathway, and the colour indicates the significance, with adjusted P-value < 0.05 deemed as significant (the full list of significant pathways can be viewed in Supplementary Tables 2–4). The mitochondrial transcripts are excluded and numbers on the plots indicate the number of genes relevant to the GO term/pathway