A novel pan-Leishmania loop-mediated isothermal amplification (Loopamp) assay for diagnosis of cutaneous and visceral leishmaniasis: a systematic review and meta-analysis

Taye, Behailu; Gebrie, Habtamu; Bogale, Alayu; Getu, Eyob; Churiso, Gemechu

doi:10.1186/s12879-025-11091-2

Table 1 Characteristics of original studies

From: A novel pan-Leishmania loop-mediated isothermal amplification (Loopamp) assay for diagnosis of cutaneous and visceral leishmaniasis: a systematic review and meta-analysis

Author, Year	Country of Patients	Design	n	Sex^a (%)	Mean age	Leishmania species	Case	Reference	Sampling	DNA Isolation	TP	FP	FN	TN
Adams, 2018 [32]	Ethiopia	C-s	50	UK	UK	L. donovani^c	VL	Microscopy	WB	Qiagen	24	0	2	24
Adams, 2018 [32]	Colombia	C-s	105	25.7	23	L.(v.) panamensis^b	CL	Microscopy	SS	Qiagen	76	3	4	22
Mukhtar, 2018 [34]	Sudan	C-s	185	37.9	UK	L. donovani^c	VL	Microscopy	WB	Qiagen	84	1	0	100
Mukhtar, 2018 [34]	Sudan	C-s	185	37.9	UK	L. donovani^c	VL	Microscopy	WB	B&S	82	1	2	100
Ibarra-Meneses, 2018 [33]	Spain	C-s	10	UK	UK	L. infantum^c	VL	PCR	WB, BM	Qiagen	6	0	0	4
Ibarra-Meneses, 2018 [33]	Spain	C-s	10	UK	UK	L. infantum^c	VL	PCR	WB, BM	B&S	6	0	0	4
Vink, 2018 [44]	Afghanistan	C-s	274	52.2	20	L. tropica^b	CL	Microscopy	SDB	Qiagen	186	48	18	22
Vink, 2018 [44]	Afghanistan	C-s	274	52.2	20	L. tropica^b	CL	PCR	SDB	Qiagen	237	1	15	21
Schallig, 2019 [45]	Suriname	C-s	93	7.5	33.6	L. (v.) guyanensis^b	CL	Microscopy	SDB	Qiagen	67	8	12	6
Schallig, 2019 [45]	Suriname	C-s	93	7.5	33.6	L. (v.) guyanensis^b	CL	PCR	SDB	Qiagen	74	1	7	11
Hagos, 2021 [46]	Ethiopia	C–c	114	4.9	23	L. donovani^c	VL	Microscopy	WB	B&S	82	16	5	11
Hagos, 2021 [46]	Ethiopia	C–c	274	4.9	23	L. donovani^c	VL	rk39-RTD	WB	B&S	113	5	7	149
Ibarra-Meneses, 2021 [47]	Spain	C-s	130	UK	UK	L. infantum^c	VL	PCR	WB, BM	Qiagen	84	2	1	43
Ibarra-Meneses, 2021 [47]	Spain	C-s	100	UK	UK	L. infantum^c	CL	PCR	SB	Qiagen	56	0	0	44
Hossain, 2021 [36]	Bangladesh	C–c	160	41.9	UK	L. donovani^c	VL	rk39-RTD	WB	Qiagen	76	0	4	80
Hossain, 2021 [36]	Bangladesh	C–c	160	41.9	UK	L. donovani^c	VL	rk39-RTD	WB	B&S	77	0	3	80
Hagos, 2024 [48]	Ethiopia	C-s	226	6.4	24	L. donovani^c	VL	PCR	WB	B&S	135	2	8	81
Taye, 2024 [49]	Ethiopia	C-s	122	39.3	22	L. aethiopica^c	CL	Microscopy	TD	Maxwell	31	7	33	51

^a% of female patients, ^bpredominant species confirmed by PCR, ^cspecies indicated, n: sample size, TP true positive, FP false positive, TN true negative, FN false negative, B&S boil-and-spin, BM bone marrow aspirates, SB skin biopsy, SDB skin-dental broach, SS skin-swab, TD Tape-disc, WB whole blood, UK unknown, C–c case–control, C-s cross-sectional, PCR polymerase chain reaction, rk39-RDT recombinant antigen 39 rapid diagnostic test, CL cutaneous leishmaniasis, VL visceral leishmaniasis

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ISSN: 1471-2334

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